Year: 2014 | Month: July | Volume 7 | Special Issue
Biological removal of synthetic textile dye Reactive Red M5B by isolated white rot fungal culture AGYP-1 under optimized culture conditions
Abstract:
The contamination of soil and water by dye containing effluents is the major and most important environmental problem. The removal of 10 different synthetic textile dyes using white rot fungal isolate AGYP-1 was investigated. The screening of decolorization using solid and liquid media demonstrated an effective removal of Reactive Red M5B by the isolate. Laccase and MnP were found to be major enzymes involved in the decolorization. The dye decolorization efficiency of the isolate was further improved by optimizing various physico-chemical parameters. The isolate was capable to decolorize 100 mg l-1 dye at pH 5.0 and 30oC under shaking condition. The supplementation of maltose (20 g l-1) and gelatine (2.5 g l-1) improved the decolorization rate by 1.6 times along with 10.28 and 18.66 times higher production of laccase and MnP. A significant decolorization of 500 mg l-1 of the dye was achieved by the isolate AGYP-1. The degradation of Reactive Red M5B was confirmed by Uv-visible spectrophotometric and HPTLC analysis. This suggests the potential application of the isolate AGYP-1 for the treatment of dye containing industrial effluents.
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